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AIM: To study the protective effects of baicalin on myocardial ischemia-reperfusion injury in rats. METHODS: The models of myocardial ischemia-reperfusion injury were established by occluding left anterior descending coronary artery (LAD) for 30 min, followed by reperfusion for 120 min. The rate of rise and decline of left ventricular pressure (±dp/dtmax) and end-diastolic pressure of left ventricle (LVEDP) were monitored continuously with polygraph. After reperfusion, the blood and myocardium samples were taken for determination of malondialdehyde (MDA) content, superoxide dismutase (SOD), Na+-K+-ATPase, Ca2+-ATPase activities in myocardium, creatine kinase (CK) and lactate dehydrogenase (LDH) in serum with spectrophotometer. The ultrastructural changes in ischemic myocardium were assessed by transmission electron microscope. RESULTS:dtmax and LVEDP, decreased plasma CK and LDH levels, reduced myocardial MDA content, and increased the activities of SOD, Na+-K+-ATPase, and Ca2+-ATPase in myocardium following ischemia-reperfusion. The ultrastructural injury in reperfused myocardium was relieved. CONCLUSION: Baicalin possesses a protective effect against myocardial ischnemia-reperfusion injury through scavenging oxide radicals and improving Na+-K+-ATPase and Ca2+-ATPase activities.
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AIM To investigate the mechanism of anti-tumor effects of curcumin on human SPC-A1 cells. METHODS Cell culture、fluorescence microscope、TUNEL method and irradiation immunity techniques were used. RESULTS ①By the action of curcumin, SPC-A1 cells grew against the wall and then peeled off gradually;fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different sizes; The TUNEL method further certified that apoptotic cells reached 42 67% at the concentration of 20 ?mol?L -1 curcumin at 24 h. ②The level of intracellular cAMP concentration in the SPC-A1 cells,treated with curcumin, was significantly higher than that of the control group. ③Treated with 20 ?mol?L -1 curcumin for 24 h, the expression of Caspase-8 mRNA of human SPC-A1 cells was up-regulated. CONCLUSION Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanism may be associated with the rising of the level of intracellular cAMP concentration, and up-regulating expression of Caspase-8.
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<p><b>OBJECTIVE</b>To explore the effects of fluid resuscitation during the shock stage of mass burn patients.</p><p><b>METHODS</b>Eleven major burn patients out of 25 were rescued at spot and were resuscitated. The treatment results were analyzed and concluded.</p><p><b>RESULTS</b>The eleven patients were all cured by smoothly living through the shock stage.</p><p><b>CONCLUSION</b>The approaching of medical personnel to the site might be the guarantee of correct and timely fluid resuscitation during shock stage, which could be effective on the prevention of burn shock development.</p>
Subject(s)
Adult , Humans , Burns , Therapeutics , Resuscitation , Shock , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of tourniquet in burn patients during tangential excision on the extremities.</p><p><b>METHODS</b>Seventy - nine burn patients who were arranged to receive tangential excision and skin grafting on the extremities were randomly divided into A and B groups. The patients in A group (n = 41) underwent the operation with the tourniquet applied continuously throughout the operation, while those in B group (n = 38), only with tourniquet applied during tangential excision. The amounts of blood loss and blood transfusion, the operation time and the take rate of grafted skin and the incidence of complications were investigated and recorded.</p><p><b>RESULTS</b>The amounts of blood loss and blood transfusion during operation in A group were 42% and 50% less than those in B group, respectively (P < 0.001). Moreover, the operation time on the upper and lower extremities in A group was much shorter (for 41% and 37%, respectively) than those in B group (P < 0.001). In addition, there was no difference of the take rate of skin graft and the incidence of subcutaneous hematoma between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>Continuous tourniquet application during tangential excision on the extremities in burn patients was proved to be effective in reducing operational blood loss, blood transfusion and in shortening operation time.</p>
Subject(s)
Adult , Humans , Blood Loss, Surgical , Blood Transfusion , Burns , General Surgery , Extremities , General Surgery , Skin Transplantation , TourniquetsABSTRACT
Objective:To study the effect of diammonium glycyrrhizinate (DG) on left ventricular function impairment during myocardial ischemia－reperfusion in rats. Methods:The left ventricular was cannulated through right common carotid artery on the myocardial ischemia－reperfusion injury model. The left ventricler function was assessed before and 10 minutes after the administration of DG, immediate and 20 minutes after initiation of ischemia, immediate and 30, 60 minutes after reperfusion, by measuring the left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), ＋dp*dtmax and －dp*dtmax. Results:Compared with the baseline, all measurements of LVSP, ＋dp*dtmax and －dp*dtmax decreased (P＜0.05,P＜0.01), while LVEDP increased (P＜0.01) after administration of DG. As the time of ischemia－reperfusion lasting, LVSP, dp*dtmax and －dp*dtmax generally decreased progressively, while LVEDP generally increased progressively. 20 minutes after the initiation of ischemia and immediate, 30 and 60 minutes following reperfusion, LVSP, ＋dp*dtmax and －dp*dtmax in the DG group were significantly higher than those in IR group (P＜0.01), LVEDP significantly lower than that in IR group (P＜0.01) at the same time. Conclusion:DG can protect left ventricular function against myocardial ischemia－reperfusion injury in rats.
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Objective To observe the effects of diammonium glycyrrhizinate (DG) on arrhythmia induced by acute myocardial ischemia-reperfusion injury.Methods Electrocardiogram of lead Ⅱwas examined on the myocardial ischemia-reperfusion model,which was induced by the 10 min of ligation of left descending coronary artery and then 30 min of reperfusion in rats.Myocardial malondialdehyde (MDA) content,superoxide dismutase (SOD) activity and adenosine triphosphatase (ATPase),and serum lactate dehydrogenase (LDH) and creatine phosphokinase (CK) levels were measured.Results As compared with the model,DG significantly reduced the incidence of ventricular arrhythmia and its score (P
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Objective To explore the developmental toxicity of tributyltin chloride and the effects on sex hormone in female offspring rats through maternal gestation exposure. Methods Pregnant Wistar rats were randomly divided into four groups, 4 in each group. They were treated with TBTC by gavage at the dose of 0, 1, 2.5 and 5 mg/kg bw respectively from days 12-20 of gestation. 10 female offspring rats were randomly selected from each group and killed on postnatal day 70. The liver, kidney, uterus and ovary were weighed and the organ indexes were calculated. Pathological examination for liver, kidney, uterus and ovary were performed. Follicle stimulating hormone(FSH), luteotropic hormone(LH), testosterone(T) and estradiol(E2) in serum was determined by radioimmunity method. Results Increase of body weight in 2.5, 5 mg/kg bw groups significantly decreased(P
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To investigate the effective principles of early management in extensively burned patients,162 patients (from 1990 to 1999) with extensive burn were treated according to the following principles: ① prompt and rapid fluid resuscitation during shock stage for delayed resuscitation patients; ② early tracheostomy,air way humidification and lavage for moderate and severe inhalation injury;③ escharectomy and grafting performed during early stage, presenting the experiences of escharectomy during shock stage in grassroot hospitals ; ④ undergoing enteral nutritional support in early stage; ⑤ emphasizing the importance of functional excise in early stage to stress that rehabilitation therapy should be implemented throughout that whole course of treatment. The results showed that the successful rate of treatment was raised and the incidence of complications and disability decreased after implementing the aforementioned treatment principles in extensively burned patients. It is suggested that the application of “Five early Principle” on extensively burned patients is effective and feasible